The national health and health commission issued a document! There has been a major adjustment in nucleic acid detection.
In view of the characteristics of Omicron mutant, such as fast spread, strong infectivity and hidden spread, the Guide (Third Edition) has made several revisions, aiming at finding infected people as early as possible in a shorter time and controlling the source of infection.
0one "All staff inspection" becomes "regional inspection"
The new edition of the Guide changes the "all staff" in the second edition to "area", and the size of the area is determined by the needs of epidemic prevention and control.
The Guide puts more emphasis on accurately delineating the scope of detection, and requires that nucleic acid detection should be targeted and targeted, and should not be flooded with water, and should not be "one size fits all" to effectively use valuable nucleic acid detection resources.
The "area" in the Guide includes sealed control area, control area and prevention area. The area may be as small as a building, or as large as the whole city. The local epidemic prevention and control headquarters should, on the basis of scientific judgment,According to the actual needs of epidemic prevention and control, it is determined and dynamically adjusted.
02 All tests must be completed within 24 hours.
The new version of the Guide clearly requires thatNucleic acid detection within the designated area should be completed within 24 hours.
In principle, all sampling tasks should be completed within 6 hours; If it is really difficult, it can be completed within 12 hours.
In addition, it is necessary to strengthen the organization and mobilization of nucleic acid sampling, and strive for the people to queue for no more than 20 minutes to avoid cross-infection. Separate detection channels for "code yellow" personnel.
0three The sampling mode changes, and five-in-one mixed mining is no longer adopted.
The new edition of the Guide cancels the 5-for-1 and adds 20-for-1.It is required to determine the sampling method according to the control measures taken by the target population:
1. Centralized isolation points and other key populations shall be subject to single mining and single inspection.
2. The people in the closed control area shall be subject to single sampling and single inspection or one household and one tube.
3. 10-in-1 mixed mining can be implemented in the control area.
4. Prevention areas and other areas can implement 20-in-1 mixed mining.
5. In the case that the single-sampling and single-inspection objects have not been detected positive in multiple rounds of testing, 10-in-1 mixed sampling can be implemented as appropriate.
04 Provide the basis for measuring the testing strength.
All districts and cities, regardless of population size, match sampling and testing strength according to the goal of completing nucleic acid testing in the whole city within 24 hours.
Calculate the number of sampling personnel according to the standard that each sampling station (2 sampling personnel) collects 120 people per hour. The calculation formula is: number of nucleic acid sampling personnel (unit: person) = population ÷ 360;
If the 10-in-1 mixed sampling detection technology is adopted, the calculation formula of detection ability is: nucleic acid detection strength (unit: tube/day) = population ÷ 10× 2;
If the 20-in-1 mixed sampling detection technology is adopted, the calculation formula of detection capacity is: nucleic acid detection strength (unit: tube/day) = population ÷20×2.
Nucleic acid result feedback needsEnsure that the results can be queried within 24 hours..
0five Patients with positive apheresis test will not be notified to designated medical institutions.
Patients with positive apheresis test will not be notified to the designated medical institutions, but will be notified to the centralized isolation management facilities to prepare to receive the positive infected persons.
The positive disposal of mixed mining test should be completed within 6 hours. Mixed sampling positive single sampling review requires double sampling of nasopharynx and oropharynx.
Figure Flow chart of positive disposal of mixed mining detection
0six Set up 9 working classes to handle special matters.
Omicron spreads fast, and the speed of the nucleic acid sampling working group is even faster. According to the links and departments involved in regional nucleic acid detection, the local authorities were instructed to set up nine work classes, including data statistics classes and sampling and matching classes, and the responsibilities of the classes were specified to avoid simply dividing the work tasks and dispatching them to counties and districts.
Nine working groups can take care of major events and small situations, and stand by in real time to serve the nucleic acid detection work.
0seven Antigen detection as a supplement, further speed up.
Promote the monitoring mode of "antigen screening and nucleic acid diagnosis", increase antigen detection as a supplementary means of regional nucleic acid detection, and organize the implementation after studying and refining the implementation plan.
Whether the task of nucleic acid detection at the grass-roots level increases or not depends mainly on how the "area" is delineated. The setting of the area size is entirely up to the local authorities, which greatly tests the local epidemic prevention level, courage and wisdom.
08 Strengthen quality control, and set up a casing supervision mechanism.
The nucleic acid sampling supervision team guides each sampling point in the form of slicing and contracting, and regulates the sampling operation, personal protection and cross-infection prevention and control.
The nucleic acid detection supervision team established a squatting supervision mechanism and organized clinical inspection experts.Point-to-point supervision of each testing institution.
The following is the original text of the Guide.
Guidelines for the implementation of regional novel coronavirus nucleic acid detection organizations
(third edition)
In order to further guide all localities to do a good job in the prevention and control of the epidemic situation in COVID-19, ensure the completion of regional nucleic acid detection tasks with good quality and quantity within the specified time, and realize "early detection, early isolation, early diagnosis and early treatment", combined with previous practice, we revised the "Guidelines for the Implementation of Nucleic Acid Detection Organizations in novel coronavirus (Second Edition)" and formed the "Guidelines for the Implementation of Nucleic Acid Detection Organizations in novel coronavirus (Third Edition)".
I. General requirements
On the basis of accurate and rapid epidemiological investigation and community control, scientifically judge the risk of epidemic spread, delimit the scope of regional nucleic acid detection, formulate specific work plans, strengthen organization and management, optimize the process of collection, delivery, inspection and reporting, and further improve the quality and efficiency of nucleic acid detection. After the outbreak of COVID-19, the districts and cities where it is located, including megacities with a permanent population of more than 10 million, should complete the regional nucleic acid detection task within 24 hours. When necessary, it can be guaranteed through regional collaborative support; If necessary, you can apply for national support.
Second, organization and management
(1) Establish an organizational mechanism.Districts and cities set up a working group for nucleic acid detection under the local joint prevention and control mechanism, led by the Standing Committee of the Municipal Party Committee, and organized relevant departments such as health, public security, civil affairs, transportation, finance, industry and information technology, ecological environment, and propaganda departments of the Party Committee to establish a flat working mechanism, which will be urgently activated when an epidemic occurs. Districts and cities will coordinate the regional nucleic acid detection work as a unit, grasp the overall situation of the jurisdiction, and avoid simply dividing the work tasks and dispatching them to counties and districts.
(two) to formulate the work plan.Districts and cities shall formulate an operable regional nucleic acid detection work plan under the local joint prevention and control mechanism, clarify the division of responsibilities and responsible persons of relevant departments, straighten out the whole chain workflow, combine emergency with emergency, and carry out early warning screening drills in a timely manner.
(three) the establishment of special classes.
1. Special class of data statistics.Mainly responsible for the centralized management of data information generated by regional nucleic acid detection, establishing a clear information flow process, and collecting, counting and reporting by special personnel. The nucleic acid detection working group sends a resident contact person to each detection institution that undertakes the regional nucleic acid detection task, establishes a contact supervision mechanism between the data statistics special class and the detection institutions, grasps the detection progress of each institution in real time, solves the problems encountered at any time, and urges the detection results to be uploaded in time.
2. Sampling and inspection matching special class.Mainly responsible for making the matching plan of sampling and inspection according to the work plan of nucleic acid detection, and strengthening the dynamic command and adjustment of sampling and inspection matching. It is necessary to accurately grasp the nucleic acid detection capacity that can be used to mobilize the whole city and the population base of each street (township), community and community. In the process of regional nucleic acid detection, it is necessary to grasp the progress of collection, delivery and detection in real time to avoid uneven busy and idle time of testing institutions or backlog of samples.
3. Special class for sample transfer.It is mainly responsible for scientific calculation of transportation capacity demand, rational deployment of transshipment force, close cooperation with the sampling and inspection matching class, and dynamic adjustment of sample transshipment scheme to ensure that all testing institutions enter the saturated working state at the fastest speed. The arrangement and deployment of transportation capacity should be optimized according to the number and distance of nucleic acid sampling points, the carrying capacity of transfer vehicles and the time required for transfer.
4. Special class for handling positive results.Mainly responsible for the overall management of the disposal of all positive results of nucleic acid detection in epidemic-related cities (including positive results of apheresis and positive results of mixed apheresis), and quickly coordinate the completion of information flow, review of apheresis positive and transfer of apheresis positive personnel. The special class consists of 120 transport, public security, disease control and emergency sampling teams.
5. Material support special class.Mainly responsible for the estimation, storage and supply guarantee of all kinds of materials needed for regional nucleic acid detection, including detection materials, protective materials, transshipment materials, disinfection materials and necessary living materials, etc., and establish an effective calling mechanism for convenient management nearby. Among them, the consumables required for sampling are stored locally according to the local population, and the cities and counties are uniformly dispatched.
6. Special quality control class.Mainly responsible for the quality control of regional nucleic acid detection, set up nucleic acid sampling supervision team and nucleic acid detection supervision team to supervise and supervise the operation of sampling personnel, personal protection, quality control of laboratory testing and prevention and control of cross-infection in sampling points and laboratories. By strengthening quality control, we can minimize false negatives or false positives and put an end to the phenomenon of issuing false nucleic acid test reports.
7. Special medical waste disposal class.Mainly responsible for the management of collection, temporary storage, transportation and disposal of medical wastes generated in all aspects of nucleic acid detection, timely adjust the frequency of removal and centralized disposal of medical wastes according to the increase or decrease of medical wastes.
8. Special information security class.Responsible for the operation and monitoring of the nucleic acid detection information system, timely handling information system failures, ensuring the safe operation of the system and avoiding problems such as downtime or system collapse; Do a good job in communication guarantee during nucleic acid detection.
9. Comprehensive management class.Mainly responsible for writing the regional nucleic acid detection work plan, nucleic acid detection analysis report, and the comprehensive coordination and guarantee of each work class in the nucleic acid detection working group.
According to the actual situation, all localities can add or merge the above nine special classes according to the organization and mobilization, sample collection, specimen detection, order maintenance, biosafety, supervision and inspection, news propaganda, etc. required for the work, but the responsibilities must be clear and implemented to all people, and there is no responsibility gap.
Third, the work content
(1) Find out the population base.All districts and cities implement the "four-party responsibility", establish work accounts, and find out the actual population base of streets (towns), communities and communities within their jurisdiction through grid management and dragnet investigation.
(2) Measuring the sampling and detection capability.All districts and cities, regardless of population size, match sampling and testing strength according to the goal of completing nucleic acid testing in the whole city within 24 hours. Among them, in principle, all sampling tasks should be completed within 6 hours; If it is really difficult, it can be completed within 12 hours. Calculate the number of sampling personnel according to the standard that each sampling station (2 sampling personnel) collects 120 people per hour. The calculation formula is: number of nucleic acid sampling personnel (unit: person) = population ÷ 360; If the 10-in-1 mixed sampling detection technology is adopted, the calculation formula of detection ability is: nucleic acid detection strength (unit: tube/day) = population ÷ 10× 2; If the 20-in-1 mixed sampling detection technology is adopted, the calculation formula of detection capacity is: nucleic acid detection strength (unit: tube/day) = population ÷20×2.
(3) Refine the sampling scheme.
1. Sampling point setting.The first is to scientifically plan sampling points. Each district and city shall comprehensively plan the layout of sampling points according to the population, geographical transportation and the distribution of nucleic acid testing institutions. According to the sampling time of 6 hours per day, a sampling point can be set with reference to 2,000-3,000 people and a sampling station for 600-800 people, and each sampling point needs 4-5 sampling stations (all localities can make overall plans according to the actual population of communities, streets, towns, rural areas and suburbs). In principle, sampling points can be set in units of communities. The second is to select sampling points scientifically. The sampling point should be an open, well-ventilated and relatively independent site, and venues such as gymnasiums, exhibition halls and school playgrounds with ventilation and independent space can be selected. Outdoor sampling points are encouraged when natural conditions permit. The third is to scientifically arrange sampling points. The sampling point is divided into waiting area, sampling area, buffer area, temporary isolation area and medical waste temporary storage area, effectively dispersing the density of people to be detected. Set up a protective clothing wearing area, equipped with hand hygiene facilities, mirror or protective device. Clear guiding signs should be set up at sampling points to ensure one-way flow of personnel, and the sampling process and matters needing attention should be defined. Groups over 60 years old, pregnant women and disabled people should set up green channels for independent sampling. A special sampling point (sampling channel) for nucleic acid detection of health code "yellow code" personnel should be set to avoid infection caused by personnel crossing. The fourth is to quickly build sampling points. The sampling points are required to complete the specification construction and activation within 2-4 hours, and on the basis of fixed sampling points, the grid management mode is adopted, focusing on community sampling.Refine and improve the layout by entering schools, enterprises and units, so as to facilitate the sampling of the masses and improve the sampling efficiency.
2. Sampling organization.On the basis of grid management, strengthen the organization and mobilization of nucleic acid sampling, clarify the departments and personnel responsible for organization and mobilization, and standardize the organization and mobilization workflow. Take residential buildings, natural village groups, schools, institutions, enterprises, companies, markets, hotels, etc. as the smallest units, so as not to lack households and people. Strengthen the fine organization and management of nucleic acid sampling site. While maintaining the work order by staff and volunteers, we encourage the use of physical means such as chairs and metal grid lines to strictly implement the "1-meter-long" interval requirements. By making an appointment, notifying and sampling in advance, we will reduce the gathering of people in a short period of time, and strive to keep the people in line for less than 20 minutes to avoid cross-infection.
3. Sampling method.Determine the sampling method according to the control measures taken by the target population. Centralized isolation points and other key populations are subject to single mining and single inspection; The people in the closed control area shall be subject to single sampling and single inspection or one household and one tube; 10-in-1 mixed mining can be implemented in the control area; Prevention areas and other areas can implement 20-in-1 mixed mining. In the case that the single-sampling and single-inspection object fails to detect positive after several rounds of testing, 10-in-1 mixed sampling can be implemented as appropriate.
(4) Implement the sampling power.Prepare sampling medical personnel and information entry personnel in advance, and carry out relevant training. On the basis of reasonable assessment of workload, staffing is required. Each sampling station should be equipped with 2 sampling personnel (medical personnel, considering shift change) and 1 information entry personnel, and each sampling station should collect 120 samples every hour. Sampling medical staff should be familiar with the collection methods of nasopharyngeal and oropharyngeal swabs, and carry out strict personal protection and infection control training. Information entry personnel should be familiar with the operation process of nucleic acid detection information system and carry out relevant infection control training.
(5) sample preservation and transshipment.Test samples should be kept at low temperature (2-8℃). One hour after the start of regional nucleic acid sampling, the first batch of samples should be transported to the testing institution to start the operation of the testing institution. Follow-up samples can be collected and transported once every half an hour to two hours, and the samples can be delivered to the laboratory 3 hours after collection. Give priority to the transfer of samples in the sealed control area and the control area, and ensure that they are transferred once every 2 hours; Remote villages, mountainous areas and pastoral areas shall reasonably determine the transshipment time and frequency according to specific conditions. It can be referred that the number of samples sent each time is about 10% of the single-day detection ability of the detection institution, so as to carry out the detection in an orderly manner.
According to the actual demand, the sample transfer class estimates the number of special vehicles, personnel and transfer boxes needed for transfer, and reserves and calls the vehicles, personnel and transfer boxes. At the same time, it is necessary to file with the health administrative department the situation of vehicles undertaking the task of regional nucleic acid detection and transshipment. During the transportation task, the vehicle shall not be used for other purposes, and biological safety protection shall be done during transportation. The transportation of non-inactivated samples shall be approved by the health administrative department at or above the provincial level according to law. Public security, transportation and other departments should do a good job in transportation security according to actual needs.
Do a good job in personnel protection and disinfection of articles during the transfer of nucleic acid samples. Non-inactivated samples are packaged with PI602 according to Class A infectious substances of UN2814 in the World Health Organization’s Guidelines for the Transportation of Infectious Substances, and inactivated samples are packaged with PI650 according to Class B infectious substances of UN3373. Before the sample transfer box is closed, the surface of the container must be disinfected with 75% alcohol or 0.2% chlorine-containing disinfectant.
(6) Strengthen information support.
1. Establish a nucleic acid detection information system.All provinces should coordinate the construction of regional nucleic acid detection information system in districts and cities. The information system should realize fast and accurate entry of relevant information, including the information of the examinee (name, certificate type, certificate number, residential address and telephone number) and sampling information (name of sampling point, district and county, sample number, date and time of sample collection, collection location, type and quantity), and quickly feed back the information of the examinee who is suspected to be a positive sample. Realize the rapid, accurate and real-time monitoring of information collected, sent, tested and reported in nucleic acid detection, and achieve the whole process management of nucleic acid detection. The information system can support multi-roles, multi-users and high concurrent operation, and has the function of collecting, sending and checking all kinds of information in real time. Before going online for use, stress tests should be conducted in advance to ensure the stability of the information system according to the standard of completing the sampling of 10% of the population actually managed every 10 minutes.
2. Do a good job of matching collection, delivery and inspection.Before starting the regional nucleic acid detection, the matching of collection, delivery and detection should be done well in advance, and dynamic scheduling should be carried out. First, according to the sampling ability of the set sampling points and the actual testing ability of each testing institution, the matching of dicing and sample delivery should be done in advance. Second, according to the experimental rhythm of nucleic acid detection institutions, samples are sent in batches and dynamically scheduled, so as to achieve "no empty machines and no accumulated samples" and make full use of the detection capabilities of each detection institution.
3. Feedback of nucleic acid results.All districts and cities should feedback the results of nucleic acid test in time, so as to ensure the accuracy of information in all aspects of collection, delivery and inspection, provide the public with the methods and ways to inquire about the test results in time, do a good job in the stress test of the inquiry service system in advance, and ensure that the results can be inquired within 24 hours after sampling by the masses.
(seven) to ensure the supply of materials.In accordance with the principle of "rather prepare than use, not available without preparation", before starting the regional nucleic acid detection work, we should do a good job in material support in advance. Ensure that the required sampling tubes (single tube, 10-in-1, 20-in-1, etc., inactivated sampling tubes are recommended), throat swabs (nasopharynx and oropharynx), detection reagents, medical consumables, protective articles, disinfection products and other materials are fully supplied, and the quality is excellent, the model and specifications are suitable, and office supplies, necessary daily necessities and other materials are fully supplied. The material reserve of sampling points should fully consider the factors of special weather such as rain, snow, low temperature, high fever and typhoon, and carry out inventory and ledger reserve management according to the layout of sampling points.
Fourth, scientifically determine the detection strategy
The joint prevention and control mechanism of districts and cities should judge the spread trend of the epidemic according to the traceability of the epidemic and the push of close contact personnel, and scientifically define the scope of nucleic acid detection in risk areas and regions and dynamically adjust it at the first time. In principle, in the first 3 days after the outbreak, a round of regional nucleic acid detection was carried out every day to find out the potential risks in society; After that, the scope and frequency of subsequent screening were determined according to the comprehensive judgment of the traceability of epidemic regulation, the scope of community control and the results of nucleic acid screening.
If the social risks are not controlled and the risk points are unclear, continue to carry out one round of regional nucleic acid detection every day. When the social risks are controlled and the risk points are basically found out, according to the distribution of cases, the key areas can be divided according to the relevant streets, communities and communities, and the key areas can be inspected daily, and the non-key areas can be inspected every other day or every three days according to the situation, and the test results can be issued within the specified time as required. Gradually narrow the scope of nucleic acid screening, improve the screening accuracy, scientific and accurate screening.
All localities can promote the monitoring mode of "antigen screening and nucleic acid diagnosis" in combination with the relevant requirements of antigen detection in Covid-19, increase antigen detection as a supplementary means of regional nucleic acid detection, and organize the implementation after studying and refining the implementation plan.
Five, standardize the positive sample disposal process
(1) Disposal process of positive detection of apheresis.When the single sampling test result is positive, the testing institution shall immediately report it to the data statistics class of the nucleic acid testing working group, and the data statistics class will immediately notify the positive result disposal class, which will carry out the following five tasks: First, inform the 120 negative pressure ambulance to transport the positive infected person; The second is to inform the centralized isolation management facilities to prepare to receive the positive infected person; The third is to inform the community (community) where the positive infected person is located to find and control the positive infected person; The fourth is to inform the public security organs to assist in the transfer of positive infected persons and carry out the track investigation of the positive infected persons; The fifth is to inform the disease control department to carry out follow-up work such as flow tracing, secret investigation and community control. The above-mentioned departments notified in no particular order.
(2) Disposal process of positive mixed mining test.Each city has set up an emergency sampling team, which is on standby 24 hours a day to make all preparations for departure at any time. When the mixed sampling test is positive, the testing institution shall immediately report it to the nucleic acid testing working group, and the working group shall also notify the emergency sampling team, the community (district) where the mixed sample is located and the public security organ to immediately isolate the personnel involved in the case in situ and conduct a single sampling review. The recheck of positive mixed sampling requires double sampling of nasopharynx and oropharynx. The rechecked samples of apheresis shall take the first inspection responsibility system and be sent to the original laboratory for testing. If the rechecked results of apheresis are positive, they shall be treated according to the treatment process of apheresis detection. The positive disposal of mixed mining test should be completed within 6 hours (see the figure below for details).
Figure Flow chart of positive disposal of mixed mining detection
Six, strengthen the quality control of nucleic acid detection
(1) Strengthen sampling quality control.The nucleic acid sampling supervision team guides each sampling point in the form of piecemeal contract, standardizes sampling operation, personal protection and cross-infection prevention and control, guides each sampling point to maintain the order on site, strengthens the preservation management of collected samples, and ensures high quality and high efficiency of sampling work.
(2) Strengthen testing and quality control.The nucleic acid detection supervision team established a squatting supervision mechanism and organized clinical laboratory experts to conduct peer-to-peer squatting supervision on various testing institutions. Laboratory performance verification, indoor quality control, and interventricular interstitial evaluation shall be carried out in accordance with the relevant requirements of the Manual for Nucleic Acid Detection in novel coronavirus of Medical Institutions (Trial Second Edition) to ensure the detection quality. Where the 20-in-1 mixed sampling test is adopted for the first time, the training and implementation should be strengthened in accordance with the Technical Specification for 20-in-1 Mixed Sampling Test of Nucleic Acid in Covid-19. Strict implementation of laboratory access and personnel access requirements, the development of laboratory standardization process, to prevent laboratory pollution and cross-infection in the laboratory.
Seven, standardize personnel and other related management
(1) Standardize the biosafety management of personnel and laboratories.All localities should, in accordance with the Biosafety Law, Regulations on Biosafety Management of Pathogenic Microorganism Laboratories, Guidelines on Biosafety of novel coronavirus Laboratories (Second Edition) and other regulations, make laboratory records as required, strengthen laboratory biosafety management, and prevent biosafety incidents. Strengthen the infection control training of medical personnel and other non-medical personnel, and the application training of regional nucleic acid detection information system. The protection requirements for sampling personnel and laboratory testing personnel shall be implemented in accordance with the Manual for Nucleic Acid Detection in novel coronavirus of Medical Institutions (Trial Second Edition). The local authorities should do a good job in overall coordination, strengthen care for the personnel involved in nucleic acid sampling and testing, and do a good job in logistics support.
(2) Strengthen the treatment of medical wastes.All localities should standardize the management of medical waste, do a good job in the collection, packaging, harmless treatment, temporary storage, handover and transfer of medical waste, use double-layer packaging bags to hold medical waste, effectively seal it, ensure that the sealing is tight, and ensure that the packaging of medical waste is not damaged or leaked. The special class for medical waste disposal should coordinate the medical waste disposal units with corresponding qualifications in time to dispose of the medical waste at the transfer sampling points and nucleic acid detection institutions. Medical wastes generated at nucleic acid sampling points shall be removed on the same day; When the temporary storage conditions permit, the temporary storage time of medical wastes generated by nucleic acid testing institutions shall not exceed 2 days. When the amount of medical wastes increases sharply and cannot be safely stored, it shall be removed on the same day. Nucleic acid detection institutions should make an appointment with the collection and transportation unit for removal time according to the temporary storage places and medical waste storage conditions, and the removal should avoid strong winds and thunderstorms. Collection and transportation units should optimize the dispatching of transport vehicles, rationally arrange the collection and transportation routes, and do a good job in ensuring the removal and transportation of medical wastes.
Key points of revision of the implementation guide of regional novel coronavirus nucleic acid detection organization (third edition)
In order to further guide local governments to do a good job in regional novel coronavirus nucleic acid detection, on the basis of fully summing up practical experience, the comprehensive group of joint prevention and control mechanism of the State Council revised and improved the Implementation Guide for All novel coronavirus Nucleic Acid Detection Organizations (Second Edition) and formed the Implementation Guide for Regional novel coronavirus Nucleic Acid Detection Organizations (Third Edition) (hereinafter referred to as the Guide).
The third edition of the Guide pointed out that on the basis of accurate and rapid epidemiological investigation and strict community control, the risk of epidemic spread should be scientifically judged, the scope of regional nucleic acid detection should be delineated, and specific work plans should be formulated to further improve the quality and efficiency of nucleic acid detection. The key revisions are as follows:
First, it emphasizes scientific accuracy and carries out nucleic acid detection in designated areas.In order to further improve the level of scientific and accurate prevention and control, the third edition of the Guide puts more emphasis on accurately delineating the scope of detection on the basis of scientifically judging the epidemic situation, and changes the "all staff" in the second edition of the Guide to "regions". The size of the regions is determined by the needs of epidemic prevention and control, and guides all localities to complete nucleic acid detection in the designated regions.
The second is to further clarify the time limit for completion.In order to adapt to the rapid spread of Omicron and other Covid-19 mutant strains, it is stipulated that the nucleic acid detection within a defined range should be completed within 24 hours, so as to further improve the ability of "early detection" of the epidemic.
The third is to add a number of special work classes.According to all the links and departments involved in regional nucleic acid detection, the local authorities were instructed to set up nine work classes, including data statistics classes and sampling matching classes, and the responsibilities of the classes were stipulated. The nine special classes are not required to be established, and all localities can flexibly add or merge according to the actual situation, but their responsibilities should not be vacant.
The fourth is to provide the basis for measuring the testing strength.In order to facilitate local calculation and preparation of detection strength, based on fully summarizing the experience of Xi ‘an and Tianjin, the number of sampling personnel and nucleic acid detection ability required to complete nucleic acid detection within 24 hours in various places are given clear calculation guidance.
The fifth is to streamline the technical requirements for testing.In combination with the application scope and actual operation requirements of this Guide, the existing requirements in technical documents such as the Manual for Nucleic Acid Detection in novel coronavirus of Medical Institutions (Trial) are not stated in this paper. Instead, the management requirements for sampling organization, transshipment, detection and result disposal are further highlighted and clarified.
Answers to Questions about the Implementation Guide of Regional novel coronavirus Nucleic Acid Detection Organization (Third Edition)
Qone
There have been two editions of "Guide" for organizing nucleic acid testing in novel coronavirus. Why should the third edition be revised and issued?
A
The first two editions of "Guidelines" have played a very good guiding role in guiding local governments to carry out large-scale nucleic acid detection. However, the situation of epidemic prevention and control in China is severe and complicated recently, and there have been cases of cluster epidemics in several provinces at the same time, or epidemics in several cities in a province at the same time. In addition, Omicron mutant strains have the characteristics of fast spread, strong infectivity and hidden spread, which puts forward new and higher requirements for organizations to carry out nucleic acid screening. At the same time, in order to summarize and popularize the useful experience of nucleic acid screening in Zhengzhou, Xi ‘an, Tianjin and other cities in the early stage and guide local governments to solve problems encountered in practice, it is also necessary to revise and form a new version of the Guide.
Q2
What are the main contents of the third edition of "Guide" compared with "Guide for the Implementation of novel coronavirus Nucleic Acid Testing Organization for All Staff (Second Edition)"?
A
Mainly revised the following aspects:
First, it emphasizes scientific accuracy and carries out nucleic acid detection in designated areas.The "all staff" in the second edition of the Guide is changed to "area", and the size of the area is determined by the needs of epidemic prevention and control.
The second is to further clarify the time limit for completion.The nucleic acid detection with defined scope shall be completed within 24 hours.
The third is to add a number of special work classes.According to all the links and departments involved in regional nucleic acid detection, the local authorities were instructed to set up nine work classes, including data statistics classes and sampling matching classes, and the responsibilities of the classes were stipulated. The fourth is to provide the basis for measuring the testing strength. The number of sampling personnel and nucleic acid detection ability required to complete nucleic acid detection within 24 hours in various places are given clear calculation guidance. The fifth is to streamline the technical requirements for testing. Combined with the application scope and actual operation requirements of the third edition of the Guide, the existing requirements in the technical documents such as the Manual for Nucleic Acid Detection in novel coronavirus of Medical Institutions (Trial) are not stated in this paper, but the management requirements such as sampling organization, transportation, detection and result disposal are further highlighted and clarified.
Qthree
What exactly does the "area" in the third edition of the Guide mean? Is it a closed control area, a control area, a prevention area, or all three areas?
A
The "area" in the Guide includes sealed control area, control area and prevention area. The area may be as small as a building or as large as the whole city, which should be determined by the local epidemic prevention and control headquarters on the basis of scientific research and according to the actual needs of epidemic prevention and control, and adjusted dynamically.
Qfour
Compared with the previous edition of the Guide for nucleic acid detection, the new edition of the Guide puts more emphasis on accurately delineating the detection range. What is the main reason for this? Will there be a large-scale full-scale inspection in the future?
A
Recently, the General Secretary presided over the Standing Committee of the Political Bureau of the Communist Party of China Central Committee Conference, emphasizing the need to improve the level of scientific and precise prevention and control. This is very important for nucleic acid detection. We require that the nucleic acid detection should be clear and targeted, and we should not engage in flood irrigation or "one size fits all" and make effective use of valuable nucleic acid detection resources. Therefore, the third edition of "Guide" not only guides all localities on how to complete the testing task with good quality and quantity within the specified time, but also emphasizes the need to do accurate and rapid flow investigation and strict community control, and delimit the scope of testing after scientific research and judgment. The scope of nucleic acid detection is directly related to whether the risk source is clear or not, and also related to many factors such as whether the traceability of the epidemic is accurate, whether the infected people are widely distributed, and whether the isolation control measures are implemented. Whether it is necessary to carry out large-scale full-scale nucleic acid detection should depend on scientific research and serve the needs of epidemic prevention and control.
Qfive
There are many links and departments involved in carrying out regional nucleic acid testing. What are the key links worthy of attention?
A
To carry out regional nucleic acid detection, local party committees and governments must have strong organizational mobilization and deployment capabilities, and all departments should cooperate efficiently. Therefore, in the Guide, we have made key requirements for the part of "organization and management". In addition to organization and management, the local authorities should fully understand and prepare for the ability of nucleic acid detection in the region. Orderly organization of community sampling, information management of sampling detection, accuracy of sampling operation, avoidance of possible cross-infection during sampling, matching of sampling and inspection, accuracy of nucleic acid detection and scientific judgment after each round of detection are all key links that must be paid attention to.
Qsix
Recently, the "Covid-19 Antigen Testing Application Scheme (Trial)" was issued. Will antigen testing be used in the process of tissue testing in various places?
A
In the third edition of the Guide, antigen detection is mentioned about the detection strategy. It is pointed out that all localities can promote the monitoring mode of "antigen screening and nucleic acid diagnosis" according to the relevant requirements of antigen detection in Covid-19, increase antigen detection as a supplementary means of regional nucleic acid detection, and organize implementation after studying and refining the implementation plan.
Content source: National Health Commission;








































